RP: Microbiology

What type of E. coli strain is used in the microbiology practical?

• Pathogenic strain

• Engineered strain

• Non-pathogenic strain

• Mutant strain

Why is it important to use a Bunsen burner during the inoculation process?

• To cool the samples

• To heat the samples

• To measure the bacteria

• To create a sterile environment

What is the purpose of flaming the lid of the bottle containing bacteria?

• To measure the amount of bacteria

• To sterilize the lid

• To cool down the lid

• To open the lid easily

What is the role of the Bunsen burner's blue flame in the experiment?

• To signal the start of the experiment

• To sterilize the surrounding area

• To increase the temperature

• To provide light

How should the bacterial culture be spread on the agar plate?

• Randomly

• Evenly across the entire surface

• Only in the center

• In a grid pattern

What indicates the effectiveness of the antibacterial agents in the experiment?

• Bubbles on the plate

• Clear areas around the disks

• Growth of bacteria throughout the plate

• Color change of the agar

What is the main reason for sealing the agar plate with tape?

• To allow oxygen to enter

• To prevent oxygen from entering

• To enhance bacterial growth

• To prevent contamination

Why should the agar plates be stored upside down after inoculation?

• To allow better visibility

• To prevent condensation on the agar surface

• To prevent the disks from falling off

• To increase air flow

Which antibacterial agent showed the largest clear area indicating high effectiveness?

• Commercial antiseptic

• Tea tree oil

• Streptomycin

• None, they were all equally effective

What does the presence of clear rings around the disks on the agar plate indicate?

• Contamination

• Effective antibacterial agents

• Ineffective antibacterial agents

• Insufficient bacterial culture