Transcription: Promoter Recognition

What is the role of promoter recognition in transcription?

• Initiating the transcription process by binding RNA polymerase to the promoter region of DNA.

• Promoter recognition leads to DNA replication instead of transcription.

• Promoter recognition is responsible for splicing mRNA.

• Promoter recognition occurs after transcription is completed.

Which specific sequence in DNA is recognized by RNA polymerase during promoter recognition?

• ATG box

• GC-rich box

• CAAT box

• TATA box (TATAAT box)

What are the key proteins involved in promoter recognition?

• Ribosomal proteins, DNA ligase, helicase

• DNA polymerase, histones, helicase

• Transcription factors, RNA polymerase, general transcription factors

• RNA helicase, DNA ligase, histones

Explain the process of promoter recognition by RNA polymerase.

• RNA polymerase recognizes the promoter region on DNA through specific sequences like the TATA box and regulatory elements.

• The promoter region is identified by the presence of enhancers

• Promoter recognition is mediated by DNA helicase

• RNA polymerase recognizes the promoter region through random binding

How does the sigma factor contribute to promoter recognition?

• The sigma factor binds to specific sequences within the promoter region of DNA.

• The sigma factor is involved in translation initiation

• The sigma factor interacts with the terminator region of DNA

• The sigma factor stabilizes the DNA double helix structure

What happens after successful promoter recognition in transcription?

• RNA polymerase dissociates from the promoter region

• Translation begins immediately

• The DNA double helix unwinds

• RNA polymerase binds to the promoter region and initiates transcription.

Discuss the significance of promoter recognition in gene expression.

• Promoter recognition is only relevant in prokaryotic cells

• Promoter recognition is essential for the initiation of transcription by RNA polymerase and the regulation of gene expression.

• Promoter recognition occurs after translation

• Promoter recognition has no impact on gene expression

What are the consequences of mutations in the promoter region on transcription?

• Altered transcription rates or inhibition of transcription

• Complete inhibition of transcription

• No impact on transcription

• Enhanced transcription rates

How can enhancers and silencers affect promoter recognition?

• Enhancers and silencers have no impact on promoter recognition

• Enhancers and silencers directly bind to the RNA polymerase

• Enhancers and silencers can affect promoter recognition by modulating the binding of transcription factors to the promoter region.

• Enhancers and silencers alter the DNA sequence of the promoter region

Compare and contrast the mechanisms of promoter recognition in prokaryotes and eukaryotes.

• Eukaryotes use RNA polymerase binding to promoter regions, while prokaryotes use transcription factors binding to enhancers and silencers.

• Eukaryotes use sigma factor binding to promoter regions, while prokaryotes use transcription factors binding to enhancers and silencers.

• Prokaryotes use sigma factor binding to promoter regions, while eukaryotes use transcription factors binding to enhancers and silencers.

• Prokaryotes and eukaryotes both use sigma factor binding to promoter regions.