3.2.3 Viral Quantification Experiment Quiz

What is the purpose of the viral quantification experiment described in the document?

• To test the effectiveness of a new laboratory equipment

• To determine the viral load of a patient at one time point using different concentrations of antiviral medication

• To compare the blood samples of different patients not receiving any treatment

• To observe the physical changes in the blood samples over time

During the plaque assay procedure, what is poured over a hard agar surface?

• A mixture of virus, bacteria, and water

• A mixture of virus, bacteria, and COLOROTP™ soft agar

• A mixture of virus, bacteria, and PBS

• A mixture of virus, bacteria, and saline solution

What is the main objective of performing a viral plaque assay in Module I?

• To determine the pH level of the sample

• To measure the size of bacteria

• To quantify the amount of virus in a sample

• To test the effectiveness of antibiotics

What is the purpose of labeling microcentrifuge tubes with "0.1", "0.01", and "0.001" in the experiment described?

• To indicate the volume of PBS added

• To indicate the dilution factor of the samples you are preparing

• To indicate the concentration of the patient sample

• To indicate the temperature at which the experiment should be conducted

According to the instructions, how should the agar plates be labeled?

• With the patient's full name and date of birth

• With the group initials, patient ID, and the time point

• With the name of the bacteria or virus being tested

• With the temperature and date of the experiment

What is the recommended method for mixing the patient sample in the dilution tubes?

• Shaking the tubes vigorously

• Using a magnetic stirrer

• Pipetting up and down or by flicking the tube

• Heating the tubes briefly

Why is it important to keep the labeled agar plates warm after preparing them?

• To prevent the agar from solidifying

• To enhance the growth of microorganisms

• Because the assay only works when the base layer is warm

• To make the labels easier to read

After adding 100 µL of the cell culture to the COLORTOP agar tube, what is the next step in the procedure?

• Quickly pour the mixture onto the agar plate.

• Add 100 µL of the patient sample (dilution factor of 1.0) to the tube.

• Incubate the plates overnight at 37°C.

• Roll the agar tube between your palms.

What should be done immediately after pouring the COLORTOP mixture onto the agar plate?

• Cover and rotate the plate.

• Stack the hardened plates.

• Label the tape with your group initials.

• Incubate the plates overnight.

How long should the soft agar overlay of all four plates be allowed to harden?

• At least 5 minutes.

• At least 10 minutes.

• At least 15 minutes.

• At least 20 minutes.