Cancer Biology_3377_Spring2024_MTT assay

What is the purpose of using PBS for filling in wells around the tested wells in cytotoxic assay?

• to control cell growth

• to prevent contamination

• to activate tested reagent

• to provide humid environment

What is the primary purpose of the MTT assay?

• To measure cell viability

• To measure DNA synthesis

• To assess protein concentration

• To determine cell motility

Which enzyme in living cells converts MTT into formazan?

• DNA polymerase

• RNA polymerase

• Mitochondrial dehydrogenases

• Lactate dehydrogenase

What is the color change observed when MTT is reduced to formazan?

• Yellow to green

• Yellow to blue

• Blue to yellow

• Colorless to red

Why are dead cells unable to convert MTT to formazan?

• They lack DNA.

• They lack active mitochondrial dehydrogenases.

• They do not take up MTT.

• They degrade MTT instead of reducing it.

At what wavelength is the absorbance typically measured in an MTT assay?

• 450 nm

• 570 nm

• 620 nm

• 750 nm