Gene and cDNA libraries

Types of gene libraries (pick more than one answers)

• cDNA

• mRNA

• Genomic

• All of the following

What is a cDNA?

• collection of total genomic DNA

• non-coding RNA molecule (containing about 22 nucleotides) found in plants,

• complementary DNA molecules synthesized from mRNA

• small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules

A gene library is a collection of restriction enzymes where each contain a portion of the genetic material of interest .

• True

• False

Where do we insert vectors?

• bacterial host cells

• restriction enzymes

• culture media

• a and b only

Below are correct statements regarding a probe EXCEPT

• A probe is used to search for its complementary sequence in a sample genome.

• The probe is labeled with a radioactive or chemical tag that allows its binding to be visualized

• A probe is a single-stranded sequence of DNA or RNA

• A probe is a double-stranded sequence of DNA or RNA

In the formation of a cDNA library, RNase used is

• RNAse H

• RNAse A

The diagram showed the steps in creating genomic library. Step 1 starts with genomic DNA extraction. Step 2 that followed would be

• Transformation into appropriate vecors

• Partial digestion with frequently used 4 base cutters

The diagram showed the steps in genomic library creation. The last step is screening for positive clones. Screening includes all the methods below EXCEPT

• colony hybridization screening with a probe

• screening on a substrate specific medium

• screening with polymerase chain reaction (PCR)

• screening using Western blotting

A genomic library can be built in a

• phage library form

• plasmid library form

• YACs library form

• All of above

Below are components/enzymes used in cDNA library construction EXCEPT

• using isolated cellular mRNA

• using genomic DNA

• using the cell's transriptome

• using reverse transcriptase