PA2 FINALS

process of extracting one material from another material by washing with a solvent (eluant) transport of solute/sample (eluent) by continuous addition of mobile phase

• Polarimetry
• Potentiometry
• Spectrometry
• Chromatography

Column chromatography and Thin layer chromatography are examples of..

• Adsorption Chromatography
• Gas-Liquid chromatography

type of partition chromatography in which the SP is a layer of water adsorbed on a sheet of paper

• Paper chromatography
• Gas chromatography
• High performance liquid chromatography

Method of choice for inorganic ions and attempts of reversed phase method are unsuccessful

• Ion exchange
• Partition Chromatography
• Adsorption Chromatography
• Size exclusion

Used in separation of solutes with different molecular size, large molecules, macromolecular complexes such as proteins and industrial polymers

• Size exclusion
• Ion exchange
• Partition Chromatography
• Adsorption Chromatography

Size exclusion chromatography used in separation of polar species

• Gel Filtration Chromatography
• Gel Permeation Chromatography

Size exclusion chromatography used in separation of non-polar species

• Gel Filtration Chromatography
• Gel Permeation Chromatography

The migration of electrically charged molecules suspended and dissolved in an electrolyte under the influence of an external electrical field (electric current)

• Electrophoresis
• Ion exchange
• Partition Chromatography
• Adsorption Chromatography

The migration of electrically charged molecules suspended and dissolved in an electrolyte under the influence of an external electrical field (electric current)

• Electrophoresis
• Ion exchange
• Partition Chromatography
• Adsorption Chromatography

widely used especially for the separation of complex mixtures of biological substances such as proteins, nucleic acids and polysaccharides

• Electrophoresis
• Ion exchange
• Partition Chromatography
• Adsorption Chromatography