Test 9 - Interference microscopy. DIC.

What is the operating principle of a transmitted light interference microscope?

• Application of diffraction gratings

• Birefringence interference

• Interference of polarized rays

• Interference between natural light and light passing through the sample

A Wollaston prism.....

• is used to compensate for polarized radiation.

• produces two rays polarised in mutually perpendicular planes exiting the prism at different angles.

• produces three beams polarized in mutually perpendicular planes.

• produces two beams, circularly polarized in different directions, at different angles.

In the differential interference contrast method, the Wollaston prism is located....

• between the crossed polarizer and analyzer at an angle of 45° to the direction of transmission of the polarizer.

• between the crossed polarizer and analyzer parallel to the transmission direction of the polarizer.

• between the parallel polarizer and analyzer at an angle of 45° to the direction of transmission of the polarizer.

A method that allows quantitative measurements to be made is....

• Differential interference contrast

• Interference microscopy

• such research is impossible

• Phase contrast

What is the maximum measurement accuracy that can be achieved in a transmitted light interference microscope?

• 10 nm

• λ/4

• λ/300

• 2λ

Why is there a dark stripe in the center of the picture located in the area where the wedges are glued together?

• The dark band is created using special inserts in the condenser to increase contrast

• The ordinary and extraordinary waves emerge with a phase difference δ = 0 and are cancelled by the analyzer

• The prism is not transparent in the visible range at the center

• A prism reflects the portion of light that passes through its center

What is common in image contrast techniques such as DIC, phase contrast and dark field?

• These are realised by the microscope's illumination system

• There is no direct impact on the object of study itself

• They produce completely identical images

• All of them produce images that are virtually free of aberrations

• None of the above

In an interference microscope (not DIC) ....

• Both beams pass through the same point of the sample

• Both beams pass through different points of the sample.

• One of the beams will pass through the sample being studied, and the second will pass by it in the surrounding environment

• One of the beams will pass to the right of the sample being studied, and the second to the left of it in the surrounding environment

Select the image that matches the differential separation

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