Test 10 - Confocal microscopy

What illumination method is used in confocal microscopy?

• Köhler illumination

• Critical illumination

• Diffused light illumination

How to increase sensitivity when scanning in confocal mode?

• Increase brightness

• Reduce noise

• Improve signal collection efficiency

• Use an objective with a larger aperture

• Increase scanning speed

Along the Z axis, the resolution of the confocal microscope ....

• is better than along the X and Y axes

• is worse than along the X and Y axes

• is the same as along the X and Y axes

• A confocal microscope does not allow information to be obtained along this axis

Select the correct statements about confocal microscopy.

• At each moment in time, the signal from the entire sample is recorded

• At each moment in time, the entire sample is illuminated

• At any given moment in time, the signal is registered only from one point.

• At any given moment in time, only one point is illuminated

When decreasing the pinhole diameter:

• There is a decrease in confocality

• The amount of transmitted scattered light is reduced

• The contrast is reduced

What units and where are used in confocal microscopy?

• Airy unit for describing the diameter of a pinhole

• Airy unit for the distance from the objective to the specimen

• Rayleigh unit for describing the thickness of an optical slice

• Rayleigh unit for determining the degree of monochromaticity of radiation

• Zeiss unit for the width of the radiation beam incident on the sample

What is NOT included in a confocal microscope?

• Traditional light microscope

• Mathematical processing algorithm

• Devices allowing to realise confocal mode

• Monochromator of incident radiation

What pinhole size is considered the limit?

• PH > 1 AU

• PH < 0,25 AU

• PH > 5 AU

• PH = 1.37 AU

How is a 3D image produced on a confocal microscope?

• A confocal microscope can only image the surface, not the volume

• When moving the scanning table along the Z axis at certain intervals

• By deflecting the laser beam to a different angle and obtaining a series of optical sections

• By gradually closing the confocal diaphragm during scanning

The pinhole is located ....

• on a plane conjugate with the plane of focus of the object.

• on a plane conjugate with the plane of the light source.

• on a plane conjugate with the rear focal plane of the lens.