Quiz on Recombinant DNA Technology

What is the role of restriction endonucleases in recombinant DNA technology?

• To cleave DNA at specific sequences

• To isolate DNA from cells

• To ligate DNA fragments

• To amplify DNA fragments

What does RFLP stand for?

• Restriction Factor Length Polymorphism

• Recombinant Factor Length Polymorphism

• Recombinant Fragment Length Polymorphism

• Restriction Fragment Length Polymorphism

Which genetically modified organism is known for its bright fluorescence?

• Suntory blue rose

• Knockout mouse

• GloFish

• Zebrafish

What is a knockout mouse?

• A mouse with enhanced gene activity

• A mouse with a gene inactivated

• A mouse that glows under UV light

• A mouse used for cloning

What is the purpose of cloning vectors?

• To carry foreign DNA into host cells

• To isolate DNA from organisms

• To degrade foreign DNA

• To amplify RNA

Which enzyme is used to join two pieces of DNA?

• Restriction endonuclease

• RNA polymerase

• DNA polymerase

• DNA ligase

What is the significance of the lacZ gene in cloning?

• It provides antibiotic resistance

• It is involved in DNA replication

• It allows for blue-white screening

• It encodes a fluorescent protein

What is the main function of PCR in recombinant DNA technology?

• To ligate DNA

• To sequence DNA

• To isolate DNA

• To amplify DNA

What type of cloning vector can hold larger DNA fragments?

• Bacterial artificial chromosomes (BACs)

• Plasmids

• Yeast artificial chromosomes (YACs)

• Bacteriophages

What is the purpose of using a selectable marker in cloning vectors?

• To degrade unwanted DNA

• To identify cells containing the vector

• To enhance gene expression

• To ligate DNA fragments