Quiz on Protein Detection and Quantitation Methods

What does the Total Nitrogen method measure?

• Only nonprotein nitrogenous compounds

• All chemically bound nitrogen in the sample

• Only total protein in plasma

• Only nitrogen in food samples

Which method is considered the classic method for quantitation of total protein?

• Kjeldahl

• Biuret

• Dye Binding

• Refractometry

What is the principle behind Refractometry?

• Measurement of color intensity

• Measurement of electric charge densities

• Measurement of refractive index due to solutes

• Measurement of protein binding to dyes

What color is formed in the Biuret method?

• Green

• Violet

• Yellow

• Blue

Which dye is most commonly used in the Dye Binding method for albumin?

• BCG

• Amido Black

• HABA

• Methyl Orange

How are proteins separated in Electrophoresis?

• By their size only

• By their electric charge densities

• By their solubility in water

• By their color intensity

At what pH do all major serum proteins carry a net negative charge?

• pH 7.0

• pH 8.6

• pH 6.5

• pH 9.0

What is the reference value range for Albumin in serum protein electrophoresis?

• 12-22%

• 7-13%

• 8-14%

• 53-65%

What is the purpose of using a cooling system in High Resolution Protein Electrophoresis?

• To enhance the color of the bands

• To maintain a stable temperature during separation

• To decrease the voltage applied

• To increase the temperature of the gel

Which method uses sodium salt to precipitate globulins?

• Dye Binding

• Electrophoresis

• Salt Fractionation

• Biuret