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Quiz on Protein Detection and Quantitation Methods

Authored by Patrick Buenaflor

Professional Development

University

Used 1+ times

Quiz on Protein Detection and Quantitation Methods
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14 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What does the Total Nitrogen method measure?

Only nonprotein nitrogenous compounds

All chemically bound nitrogen in the sample

Only total protein in plasma

Only nitrogen in food samples

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which method is considered the classic method for quantitation of total protein?

Kjeldahl

Biuret

Dye Binding

Refractometry

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the principle behind Refractometry?

Measurement of color intensity

Measurement of electric charge densities

Measurement of refractive index due to solutes

Measurement of protein binding to dyes

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What color is formed in the Biuret method?

Green

Violet

Yellow

Blue

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which dye is most commonly used in the Dye Binding method for albumin?

BCG

Amido Black

HABA

Methyl Orange

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How are proteins separated in Electrophoresis?

By their size only

By their electric charge densities

By their solubility in water

By their color intensity

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

At what pH do all major serum proteins carry a net negative charge?

pH 7.0

pH 8.6

pH 6.5

pH 9.0

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