Introductory Bacterial Conjugation

Which material is shared between two groups in the experiment?

• 3 sterile inoculating loops

• LB plate

• E. coli Strain I liquid culture

• Permanent marker

In Table 1, what symbol is used to indicate bacterial growth?

• (+)

• (-)

• (x)

• (*)

What is the first step in using a new loop and the liquid culture of Strain II?

• Place a drop of Strain II 1 inch from the drop of Strain I

• Place a drop of Strain II 1/2 inch from the drop of Strain I

• Mix Strain I and Strain II directly

• Dispose of the loop immediately.

What should be done with the loops after use?

• Wash and reuse them

• Dispose of them in a disposal container

• Leave them on the table

• Use them for another experiment.

Fill in the blank: Incubate the plates overnight at ___, or for 24–48 hours at room temperature.

• 25°C

• 37°C

• 50°C

• 100°C