Module5

1-detect chromosomal translocations: malignancies & inherited hematological d/o’s, 2- I.D. hematological infectious diseases, 3- monitor disease after cancer treatment

is from

• Molecular testing

• Flow symmetry

• WBC

• DNA alterations

DNA & RNA from the patient sample used:

• DNA more stable

• RNA more stable

From peripheral blood, tissue bx, needle aspirate, cheek swabs

• Molecular testing

• Flow Cytometry

• Electronic Impedance

Uses enzymes, DNA synthesis has many

• Copies

• Diseases

• Replications

• special heme

diseases detected by I.D.ing presence of RNA/DNA

• Probes

• Flow cytometry

• Molecular testing

• Scatter

enzyme based, DNA denatured, many cycles, primers used for specificity/accuracy

• PCR

• Probes

• Gel electrophoresis

3 control are required in the

• PCR reaction

• flow cytometry

• Southren blot

• Electrical impendence

1 is PCR controls is

• Negative

• Positive

• No DNA

Control 2 in PCR is

• Positive

• Negative

• No DNA

Control 3 is PCR is

• No DNA

• Negative

• Positive