Sb015 chapter 8

What are the tools used in recombinant DNA technology

• Plasmids, bacteriophages, cosmid, and

  YACS.

• EcoRI, BamHI, and Smal enzyme

• Target DNA, restriction enzymes, DNA

  cloning vectors, host cells, and modifying enzyme

• AmpR, tetR, kanR, and lacZ gene

What is the purpose of DNA ligase in recombinant DNA technology?

• To cut DNA at specific base restriction sites.

• To seal DNA fragments together by forming phosphodiester bonds.

• To amplify DNA sequences using PCR.

• To cleave DNA fragments containing genes of interest

What is the characteristic of plasmid as a cloning vector

• Able to accept foreign DNA in multiple cloning sites

• Able to accept recombinant DNA through transformation

• Able to express recombinant DNA

• Able to amplify the recombinant DNA

what is the characteristics of E.coli host cell

• Able to accept recombinant DNA through transformation

• Able to express gene of the recombinant DNA

• Able to amplify the recombinant DNA

• Able to maintain the structure of recombinant DNA from one generation to next

What is the function of Taq polymerase in recombinant DNA technology

• To seal DNA fragments together by forming phosphodiester bonds.

• To form phosphodiester bonds between adjacent nucleotides in DNA.

• To cut DNA at specific base restriction sites.

• To catalyze the addition of nucleotides to

  DNA sequences.

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• Insertion

• Screening

• Amplification

• Transformation

During insertion step,

• The gene of interest is inserted into a plasmid

• The recombinant plasmid is inserted into a host cell

• The plasmid is inserted into the gene of interest

Which restriction enzyme listed below was isolated from E. coli bacteria?

• EcoR1

• Sma1

Which of the following is NOT a tool used in genetic engineering?

• restriction enzyme

• plasmid

• DNA helicase

• DNA ligase

Which enzyme helps fuse the foreign into the new DNA?

• DNA Polymerase

• DNA ligase