Immuno Lab Exam 1

If you forget to wash the wells after adding the conjugate but before adding the substrate in an indirect EIA, what is the expected outcome on the color signal?

• The color will remain the same as usual.

• The color will be lower than usual.

• The color will be higher than usual.

• There will be no color development.

Why does the color intensity increase if the wash step between conjugate and substrate is skipped?

• The unbound conjugate is removed.

• The substrate is removed from the well.

• The unbound conjugate remains and reacts with the substrate.

• The enzyme activity is reduced.

In an indirect EIA, what happens if the wash step is forgotten between the primary antibody and the conjugate, but performed between the conjugate and the substrate?

• The color intensity will increase.

• The color intensity will decrease.

• The color intensity will remain unaffected.

• The test will yield no result.

Forgetting to wash after the primary antibody step in an indirect EIA results in which of the following?

• More specific binding of the conjugate.

• Increased unbound primary antibody on the solid phase.

• No effect on the assay outcome.

• Increased color signal

Tags

NGSS.HS-PS1-5

What is the primary purpose of washing steps in an indirect EIA?

• To remove unbound reagents and prevent nonspecific binding.

• To add more substrate.

• To reduce enzyme activity.

• To dilute the sample

If you forget to wash after the conjugate step in an indirect EIA, the color intensity at the end will be _______ than expected.

• higher

• lower

• the same

• unpredictable

In an indirect EIA, the washing step between the ________ and conjugate is crucial because it removes unbound antibodies which can otherwise cause nonspecific binding.

• primary antibody

• secondary antibody

• substrate

• enzyme

In an indirect EIA, would the amount of color at the end be higher or lower if you forgot the washing step between

(1) the conjugate and the addition of substrate 

(2) the primary antibody and the conjugate but remembered it between the conjugate and the substrate

• lower, lower

• higher, higher

• lower, higher

• higher, lower

The reaction between the ________ and substrate in an indirect EIA creates the color change that indicates the presence of the target analyte.

• enzyme on the conjugate

• antibody on the substrate

• buffer solution

• target analyte

In indirect EIA, the role of the conjugate is to bind to the ________ and generate a detectable signal.

• primary antibody

• secondary antibody

• substrate

• enzyme