DNA Sequencing and Biotechnology Quiz

What is the primary purpose of DNA sequencing?

• To destroy harmful DNA

• To replicate DNA strands

• To create new DNA molecules

• To determine the sequence of nucleotide bases in DNA

Which method is known as the chain termination method?

• Next-generation sequencing

• Gel electrophoresis

• Polymerase chain reaction

• Sanger sequencing

What is a unique ingredient in Sanger sequencing?

• DNA polymerase enzyme

• Fluorescent proteins

• Dideoxy nucleotides

• RNA primer

What is the function of a primer in the process of DNA replication?

• To sequence the entire genome

• To initiate the synthesis of a new DNA strand

• To bind to the DNA polymerase

• To terminate the DNA chain

What is a key advantage of next-generation sequencing over Sanger sequencing?

• It is more expensive

• It allows for highly parallel sequencing

• It uses fewer resources

• It is slower

What is the main limitation of Sanger sequencing?

• It is not accurate

• It cannot sequence individual pieces of DNA

• It is too fast for large-scale projects

• It can only sequence short DNA fragments

What is the significance of the Human Genome Project?

• It cloned human DNA

• It created new DNA technologies

• It developed new medical treatments

• It sequenced the entire human genome

What is the function of dideoxy nucleotides in Sanger sequencing?

• To terminate the DNA chain

• To sequence the entire genome

• To replicate the DNA strand

• To start the DNA chain

What is a common feature of next-generation sequencing techniques?

• They are more expensive than Sanger sequencing

• They are slower than Sanger sequencing

• They allow for sequencing reactions to take place in parallel

• They require more DNA samples

Why is fast and inexpensive sequencing important?

• It enables personalized medicine

• It allows for the destruction of harmful DNA

• It slows down research progress

• It creates new DNA molecules