Immobilization of bioreceptors

Which of the following explains why the material used to immobilise the enzyme must be inert?

• it ensures that the attachment will be strong
• it ensures that the enzyme activity is not interfered with
• it ensures that the reaction occurs faster than normal
• it prevents the death of the enzyme

Immobilised enzymes have higher thermal stability compared to free enzymes. Which of the following statements is an inaccurate interpretation of this?

• Immobilised enzymes have higher optimum temperature
• Immobilised enzymes can keep on catalysing reaction even when their free counterparts have become denatured by high temperature
• no matter how high temperature gets, immobilsed enzymes will keep on catalysing reaction
• immobilsed enzymes have a wider range of temperature within which they can act, compared to free enzymes

Analyte is a bioreceptor

• True

• False

Black Dot is ?

• Enzyme

• Antigen

• Antibody

• Analyte

Which immobilization technique involves forming bonds between bioreceptor functional groups and a support surface?

• Covalent bonding

• Physical adsorption

• Entrapment

• Cross-linking

What type of immobilization is commonly used when the bioreceptor's activity might be affected by harsh chemical reactions?

• Physical adsorption

• Covalent bonding

• Cross-linking

• Entrapment

What is the main purpose of immobilizing bioreceptors in biosensors?

• To increase bioreceptor production

• To enhance bioreceptor stability and reusability

• To decrease the bioreceptor’s sensitivity

• To reduce the bioreceptor’s lifespan

Covalent immobilization methods often require chemical reagents that may impact the bioreceptor's natural activity.

• True

• False

Physical adsorption relies on weak forces, like van der Waals interactions, to immobilize bioreceptors onto surfaces.

• True

• False

The stability of a bioreceptor is generally reduced after immobilization.

• True

• False