Electrophoresis analysis

Front

What is electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What role do enzymes play in electrophoresis?

Back

Enzymes are used to cut DNA into fragments for analysis.

Front

What happens to DNA fragments during gel electrophoresis?

Back

Smaller fragments move faster through the gel, while larger fragments move slower.

Front

What is the purpose of adding a loading dye in electrophoresis?

Back

To visualize the DNA samples and track their movement through the gel.

Front

What is agarose gel?

Back

A gel made from agarose used as a medium for electrophoresis to separate DNA fragments.

Front

What is the significance of the buffer solution in electrophoresis?

Back

The buffer maintains a stable pH and provides ions that conduct electricity during the process.

Front

What is the relationship between fragment size and migration distance in electrophoresis?

Back

Smaller fragments migrate further than larger fragments in the gel.

Front

What is a DNA ladder?

Back

A mixture of known DNA fragments used as a reference to determine the size of unknown DNA fragments.

Front

What is the purpose of staining the gel after electrophoresis?

Back

To visualize the separated DNA bands for analysis.

Front

What is the function of a power supply in electrophoresis?

Back

To provide the electrical current needed to move the DNA fragments through the gel.