Patient selection: Was a consecutive or random sample of patients enrolled?

• Yes

• No

• Unclear

Patient selection: Was a case-control design avoided?

• Yes

• No

• Unclear

Patient selection: Did the study avoid inappropriate exclusions?

• Yes

• No

• Unclear

Patient selection: Overall (high risk/low risk)

• High

• Low

• Unclear

Index Test: Were the index test results interpreted without knowledge of the results of the reference standard?

• Yes

• No

• Unclear

Index Test: If a threshold was used, was it prespecified?

• Yes

• No

• Unclear

Index Test: Overall RoB (high, low, unclear)

• High

• Low

• Unclear

Is the reference standard likely to correctly classify the target condition?

• Yes

• No

• Unclear

Were the reference standard results interpreted without knowledge of the results of the index test?

• Yes

• No

• Unclear

Reference standard: Overall RoB (high, low, unclear)

• High

• Low

• Unclear