PCR and Gel Electrophoresis
Flashcard
•
Science
•
10th - 12th Grade
•
Hard
Wayground Content
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15 questions
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1.
FLASHCARD QUESTION
Front
What is PCR?
Back
Polymerase Chain Reaction (PCR) is a technique used to amplify a specific segment of DNA, creating millions of copies of that DNA sequence.
2.
FLASHCARD QUESTION
Front
What is the role of a restriction enzyme in DNA manipulation?
Back
Restriction enzymes cut DNA at specific sequences, allowing scientists to manipulate DNA for cloning, analysis, and other applications.
3.
FLASHCARD QUESTION
Front
How many DNA copies are produced after 2 cycles of PCR?
Back
4 copies.
4.
FLASHCARD QUESTION
Front
What is gel electrophoresis used for?
Back
Gel electrophoresis is used to separate DNA fragments based on their size.
5.
FLASHCARD QUESTION
Front
What is the function of a primer in PCR?
Back
Primers are short sequences of nucleotides that provide a starting point for DNA synthesis during PCR.
6.
FLASHCARD QUESTION
Front
What is the electrical force in gel electrophoresis?
Back
The electrical force is the driving force that moves DNA fragments through the gel, separating them based on size.
7.
FLASHCARD QUESTION
Front
What happens to DNA fragments during gel electrophoresis?
Back
DNA fragments migrate through the gel matrix, with smaller fragments moving faster and farther than larger ones.
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