Omics_Day1_session3

What is the role of adding Iodoacetamide (IAA) in typical proteomics sample preparation workflow?

• Cleaves the inter and intra-chain disulphide bond

• Alkylates sulfhydryl groups of cysteine residues

• Coats the proteins with SDS for separation on the basis of molecular weight

• It ensures the protection of proteins against proteolytic enzyme

Which database focuses on the integration of metabolomic data with genomic and proteomic data to study metabolic pathways?

• KEGG

• HMDB

• LIPID MAPS

• MoNA

What are the three main steps of a typical PCR cycle?

• Denaturation, annealing, elongation

• Denaturation, transcription, translation

• Amplification, purification, sequencing

• Denaturation, ligation, amplification

Which component of the CRISPR-Cas9 system is responsible for guiding the Cas9 enzyme to the target DNA sequence?

• Cas9 protein

• Single-guide RNA (sgRNA)

• Donor DNA template

• Protospacer adjacent motif (PAM)

Which of the following factors can influence alternative splicing?

• RNA-binding proteins

• Environmental conditions

• Developmental stage of the organism

• All of the above