BLEH MOD 1

Instrument used to measure how much light a substance absorbs in consideration of wavelength

• Spectrophotometer

• Photometer

• Frequency

• Wavelength

Measures light intensity without considering the wavelength

• Spectrophotometer

• Photometer

• Frequency

• Fluorometer

Frequency is inversely proportional to wavelength. Energy is directly proportional to wavelength.

• First statement is false. Second statement is true

• First statement is true. Second statement is false

• Bothe statements are true

• Both statements are false

Answer explanation

Frequency and energy are inversely proportional to wavelength.

Energy is directly proportional to Frequency.

If the atom is at the lowest energy level, is it in ground state or excited state?

• Ground state

• Excited state

• in both state

• NOTA

Answer explanation

When the atom is at the lowest energy level it's in GROUND STATE.

If the energy is at the highest energy available it's in EXCITED STAGE.

Express the relationship between the concentration and the absorbance

• Absorbance decreases as concentration increases.
• Absorbance is directly proportional to concentration.
• Absorbance is independent of concentration.
• Absorbance is inversely proportional to concentration.

Answer explanation

ABSORBANCE and CONCENTRATION are directly proportional.

ABSORBANCE is inversely proportional to TRANSMITTED LIGHT

Light that about to strike the sample

• Reflected light

• Transmitted light
• Ambient light
• Incident light

Answer explanation

INCIDENT LIGHT- light that about to strike the sample

TRANSMITTED LIGHT- light that passes through the sample

Component of the Spectrophotometer that isolates and select specific wavelengths of light

• Prism
• Filter
• Monochromator
• Detector

Component of Spectrophotometer that converts transmitted radiant energy into an equivalent amount of electrical energy

• Optical filter
• Light source
• Spectrometer
• Photodetector

Light emitted by the Fluorometer

• Photodetector
• Laser
• Luminescence

• Fluorescence

A primary filter or excitation filter is a component of Fluorometer that absorbs light at a lower wavelength. While Secondary filter or emission filter emits light at longer wavelengths

• Both statements are true

• Both statements are false

• First statement is true. Second statement is False

• First statement is false. Second statement is true

Answer explanation

Primary Filter or Excitation filter - absorbs light at lower wavelengths (the process is called excitation)

Secondary Filter or Emission Filter - emits light at a longer wavelength (the process is called emission)