Pre Test (ISI) Modul 12 OncoGS Curriculum

Could you mention 3 types of oncoGS specimen sample?

• Liquid Biopsy, FFPE and EDTA tube

• Liquid Biopsy, FFPE and buffy coat

• Liquid Biopsy, FFPE and FFPE MSI

• Liquid Biopsy, cytology and BRCA

What's the correct general workflow of oncoGS?

• Library Preparation -> Extraction -> Hybridization -> Pooling and Sequencing -> Sample Preparation

• Sample Preparation -> Hybridization -> Extraction -> Library Preparation -> Pooling and Sequencing

• Extraction -> Library Preparation -> Sample Preparation -> Hybridization -> Pooling & Sequencing

• Sample Preparation -> Extraction -> Library Preparation -> Hybridization -> Pooling and Sequencing

What's the target of buffycoat and LB extraction in the oncoGS workflow?

• genomic DNA and DNA

• genomic DNA and cell free RNA

• genomic DNA and cell free DNA

• genomic DNA and cell free total nucleic acid

What's the reagents that we use for deparaffinization?

• Ethanol 80% and Xylene

• Xylene and ethanol 80%

• Xylene and UltraPure Water

• Xylene and ethanol absolute

What's UMI in xGen cfDNA & FFPE DNA Library Prep?

• Unique Molecular Identifiers (UMI) are short DNA that will be removed during library preparation for nextgeneration sequencing (NGS).

• Unique Molecular Identifiers (UMI) are short, random sequences of DNA or RNA that are added to individual molecules in a sample during library preparation for nextgeneration sequencing (NGS).

• Unique Molecular Identifiers (UMI) are short sequence that will be fragmented during library preparation for next-generation sequencing (NGS).

• United Molecular Identifiers (UMI) are DNA collected during library preparation for next-generation sequencing (NGS)

How long the DNA size after size selection in gDNA library preparation?

• 250 bp - 450 bp

• 200 bp - 500 bp

• 50 bp -150 bp

• 100 bp - 150 bp

What's the maximum DNA input for LB and FFPE pools in the hybridization protocol?

• 1500 ng and 1750 ng

• 1500 ng and 2000 ng

• 1500 ng and 1960 ng

• 1500 ng and 1500 ng

What's the maximum DNA input for FFPE MSI and gBRCA pools in the hybridization protocol?

• 1600 ng and 1960 ng

• 1960 ng and 1500 ng

• 2000 ng and 1600 ng

• 1960 ng and 1600 ng

What's the maximum capacity of P2 Flowcell that we use in the lab?

• 480M

• 500M

• 300M

• 700M

What's the NGS instrument name in the lab?

• Illumina NextSeq 550

• Illumina MiSeq NGS instrument

• Illumina NexSeq 2000

• NovaSeq 6000