Biotechnology 9.2 HW

What is the main purpose of using chromatography in biotechnology applications?

• To separate molecules based on size or solubility

• To combine molecules into a single phase

• To change the color of molecules

• To increase the temperature of molecules

In paper chromatography, the solvent moves up the paper by ______ action.

• gravitational

• capillary

• magnetic

• electrical

What is the purpose of the 'frit' in a chromatography column?

• To separate molecules by size

• To hold the resin in place

• To visualize molecules

• To add color to the sample.

In column chromatography, the sample is added to the top of the column and gravity pulls it through the resin bead matrix. The molecules are separated based on their size, shape, or charge. What is this process called?

• Filtration

• Dialysis

• Fractionation

• Diffusion

In the process of dialysis, a sample with a mixture of desirable and undesirable proteins is placed into a dialysis tube. What is the purpose of the dialysis membrane?

• To add color

• To separate large molecules

• To keep the protein of interest inside the bag

• To speed up diffusion.

Column chromatography involves adding the sample to the top of the column. The sample is then pulled through the resin bead matrix by gravity. This process is known as ________.

• Filtration

• Fractionation

• Dialysis

• Diffusion

Which type of chromatography separates molecules based on size?

• Ion-exchange chromatography

• Gel-filtration chromatography

• Affinity chromatography

• Hydrophobic-interaction chromatography

In gel-filtration chromatography, molecules are separated based on their ______.

• charge

• size

• shape

• hydrophobicity

What is the primary purpose of gel-filtration chromatography?

• To separate proteins based on charge

• To separate proteins based on size

• To separate proteins based on solubility

• To separate proteins based on pH.

Fill in the blank: The elution buffer in ion-exchange chromatography either contains a high salt concentration, or it has a ______ pH.

• A) neutral

• B) high

• C) low

• D) variable