Biotechnology 9.2 HW

What is the main purpose of using chromatography in biotechnology applications?

• To separate molecules based on size or solubility

• To combine molecules into a single phase

• To change the color of molecules

• To increase the temperature of molecules

In paper chromatography, the solvent moves up the paper by ______ action.

• gravitational

• capillary

• magnetic

• electrical

What is the purpose of the 'frit' in a chromatography column?

• To separate molecules by size

• To hold the resin in place

• To visualize molecules

• To add color to the sample.

In column chromatography, the sample is added to the top of the column and gravity pulls it through the resin bead matrix. The molecules are separated based on their size, shape, or charge. What is this process called?

• Filtration

• Dialysis

• Fractionation

• Diffusion

In the process of dialysis, a sample with a mixture of desirable and undesirable proteins is placed into a dialysis tube. What is the purpose of the dialysis membrane?

• To add color

• To separate large molecules

• To keep the protein of interest inside the bag

• To speed up diffusion.

Which type of chromatography separates molecules based on size?

• Ion-exchange chromatography

• Gel-filtration chromatography

• Affinity chromatography

• Hydrophobic-interaction chromatography

In gel-filtration chromatography, molecules are separated based on their ______.

• charge

• size

• shape

• hydrophobicity

Fill in the blank: The elution buffer in ion-exchange chromatography either contains a high salt concentration, or it has a ______ pH.

• neutral

• high

• low

• variable

What is the charge of the resin used in cation exchange chromatography?

• Positively charged

• Negatively charged

• Neutral

• Both positively and negatively charged.

In ion-exchange chromatography, if a protein's charge is unknown, its behavior in the column may change depending on the pH of the solution. This is because the protein's charge can be _______.

• Positive

• Negative

• Neutral

• Variable