CRISPR Cas

CRISPR is a protein whereas Cas is a DNA sequence.

• TRUE

• FALSE

Which of the following comes from viral genome?

• Palindromic repeat sequence

• Spacer sequence

• TracrRNA

• Cas gene sequence

PAM sequence is present in the target sequence that the Cas protein will cleave, not in the guide RNA.

• TRUE

• FALSE

In ​ (a)   , Jennifer Doudna and Emmanuelle Charpentier received Nobel Prize in​ (b)  

• 2020
• Chemistry
• 2019, Science
• 2021, Medicine
• 2021, Biology

NHEJ is an error-prone DNA repair mechanism that can be used to knockout a gene.

• TRUE

• FALSE

Which of the following is the PAM sequence for Cas9?

• CGG

• ATG

• ATT

• GCG

gRNA includes the following sequences-

• CrRNA

• TracrRNA

• Linker loop

• All of them