Crispr

What does the acronym CRISPR stand for?

• Controlled Reservative Image Spectacle Palindromic Recounts

• Clustered Regularly-Interspaced Short Palindromic Repeats

Tags

NGSS.HS-LS3-2

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria

Why do bacteria have CRISPR? Check all that apply.

• To be able to remember which viruses they have been infected with

• To be able to edit virus DNA

• To cut apart virus DNA thereby stopping the virus infection

• To be able to edit their own DNA

A PAM is

• a 15-20 bp sequence snipped from a viral genome that serves as a tracer DNA to locate viruses later

• a 3 bp sequence that adjacent to viral DNA in a CRISPR array

• a girl's name popular in the 1960s

• where the DNA is cut

What is the role of the guide RNA?

• It helps the cell repair the DNA

• ensures that the Cas9 enzyme cuts at the right point in the genome

• Recognizes PAM sequences and opens up the DNA

• makes a cut across both strands of the DNA

How does the guide RNA help the cell know where to cut the DNA?

• Guide RNA helps to find the PAM

• Guide RNA is complementary to the DNA sequence

• Guide RNA always binds to the same sequence of DNA

• Like restriction sites, they are always palindromes

The following sequence is a known GENE MUTATION that geneticists would like to remove. What would be the guide RNA they need to build into a CRISPR-Cas9 enzyme?

TTA CCG

• AAU GGC

• AAT GGC

• TTA CCG

• GCC ATT

Tags

NGSS.HS-LS3-2

What does the cas9 protein do?

• Prevents viruses from entering bacteria

• Cut RNA

• Cut DNA

• Binds to RNA

CRISPR-Cas9 is a type of ________ system found in bacteria.

• digestive

• endocrine

• circulatory

• immune

The enzyme portion of this complex is called ________.

• CRISPR

• Replicase

• Lysozyme

• Cas9