Blotting techniques

Probe in the Western blot is tagged with

• p32

• c14

• s35

• None of the above

Blotting is a technique to identify a

• sugar

• gene

• protein

• lipid

Why are buffers used in Electrophoresis?

• Conduct electricity

• Remove salts

• Add extension energy

• Dissolve excess DNA

In Southern blotting, ___________ type paper is used for blot transfer

• Nitrocellulose membrane or Nylon membrane filter

• Whatman filter paper

• Paper towel filter paper

• None

Which of the following is a commonly used label in blotting techniques?

• vimentin

• biotin

• avidin

• streptomycin

Liquid blot uses ------ type of transfer method

• Wet electro blotting

• Semi-dry electro blotting

• Dry electro blotting

• Southern blotting

In Dot blot & Slot blot the proteins are not separated by electrphoresis

• True

• False

Technique that employs a labeled probe for localizing specific DNA or RNA sequence targets within fixed tissues and cells is -----

• Southern blot

• Northern blot

• Western blot

• in situ hybridization

In FISH the bound probe is visualized using -----

• Light microscope

• Electron microscope

• Compound microscope

• bright-field or confocal microscopy

Which type of hybridization is used to study embryos

• Southern hybridization

• Northern hybridization

• Whole mount In situ hybridization

• In situ hybridization