Basic Microbiology Techniques Quiz

What is the primary purpose of culture media?

• To sterilize microorganisms

• To provide nutrients for the growth of microorganisms

• To identify microorganisms

• To inhibit the growth of microorganisms

Which of the following is a solid culture medium?

• Nutrient broth

• Broth

• Peptone water

• Agar

What type of media is Mannitol Salt Agar (MSA)?

• Differential Media

• Enriched Media

• General Purpose Media

• Selective Media

What is a characteristic of microorganisms?

• They have very particular growth requirements

• They grow only in solid media

• They grow in any medium

• They can survive without nutrients

Which of the following is a selective agent in Baird Parker Agar (BPA)?

• Glucose

• Phenol red

• Tellurite

• Mannitol

What does differential media do?

• Allows all bacteria to grow equally

• Produces visible changes in the growth of different species

• Only supports the growth of fungi

• Inhibits all bacterial growth

What is the function of phenol red in Mannitol Salt Agar?

• To enhance color of colonies

• To inhibit bacterial growth

• To indicate pH changes

• To provide nutrients

Which of the following is NOT a type of culture media based on physical state?

• Liquid

• Solid

• Complex

• Semi-solid

What type of media is used to select for Staphylococcus spp.?

• MacConkey Agar

• Mannitol Salt Agar

• Nutrient Agar

• Eosin methylene blue agar

What is the result of mannitol fermentation by Staphylococcus aureus in MSA?

• Yellow colonies

• Pink colonies

• Black colonies

• No color change