Spore Cycle in Microbiology

Bacteria that are metabolically active

• Vegetative cells

• Spores

Bacteria that are highly resistant and metabolically inactive

• Vegetative cells

• Spores

Layers of membrane surrounding the genetic material inside of a cell

• Endospore

• Free spore

Bacterial spores are resistant to damage by which of the following?

• Heat

• Freezing

• Radiation

• Chemicals and stains

• All answers are correct

The primary stain used during spore staining that stains the spore and not the vegetative cell

• Water

• Malachite Green

• Safranin

• Crystal Violet

Removes primary stain from the vegetative cells and removes excess stain

• Water

• Malachite Green

• Safranin

• Crystal Violet

Counter or contrasting stain used during spore staining to stain the vegetative cell

• Water

• Malachite Green

• Safranin

• Crystal Violey

Why is heat necessary in spore staining?

• To prevent the cold staining dyes from freezing the cell

• To allow the stain to penetrate the spore coat

• To differentiate between the spore and the vegetative cell

• Making sure the bacteria doesn't fall off the slide

• The spores are red; vegetative cells green

• The spores are green; vegetative cells are green

• The vegetative cells are red; spores are red

• The vegetative cells are red; spores are green

Tags

NGSS.MS-LS1-1

• These bacteria are rod-shaped bacillus

• These bacteria are circular-shaped cocci

• These bacteria are spiral-shaped spirillum

Tags

NGSS.MS-LS1-1