Optimal Conditions Chart Enzymes

Which of the following conclusions can be drawn from this graph?

• The optimum pH of the enzyme is 6.6.
  
• The optimum pH of the enzyme is 5.8
  
• The enzyme’s activity increases as pH increases 5.0 to 9.0
  
• The enzyme’s activity is greater around pH of 8.0 .
  

A change in pH can change the shape of a protein. How might a change in pH affect the function of an enzyme? 

• A new enzyme may be required to speed up the reaction.
• The enzyme may no longer be able to function for the substrate as a catalyst.
• The enzyme and substrate may no longer fit together so the enzyme cannot speed up the reaction.
• All the other answers are correct.

Enzymes are highly sensitive to pH and temperature because

• changes in the environment raise their activation energy.

• changes in temperature and pH readily break their hydrogen bonds and can alter the enzyme shape.

• environmental changes affect their primary structure.

• at extreme temperatures and pH levels, coenzymes add chemical groups to the substrate.

• extremes of temperature and pH level change the ionization rate.

What is the optimal temperature for the enzyme reaction shown? ____'c

• 10
• 20
• 40
• 60

The graph shows the effect of temperature on the activity of enzyme X.

The enzyme will be denatured at

• below 0^oC

• between 0^oC to 10^oC

• between 40^oC to 50^oC

• above 60^oC