Aseptic Technique and Performing Isolation Streaks

Which of the following instruments are used to perform a streak plate.

• Incinerating loop

• Inoculating wire loop

• Plastic loop

• Sterile Plastic spreader

Which of the following instruments are used to perform a streak plate.

• Incinerating loop

• Bacterial (e.g. E. coli) culture

• Clamp

• Stock photo

After each streak on the plate with the innoculating loop, what is done to the loop?

• Discarded

• Re-introduced to stock culture

• Flamed until loop glows

• wiped with disinfectant

What is this instrument called?

• Incinerating loop
• Clamp stick
• Inoculating loop
• Eye ring loop

What does the streak technique enable us to achieve?

• Direct spread and dilution of bacteria from a single colony

• isolation of bacterial colonies

• Visulisation of virus plaques

• development of antibacterial microbes

What type of growth medium was used for the spread technique

• Blood agar

• Nutrient agar

• Chocolate agar

• MaConkey's agar

Why were the selective media (Blood/MaConkey's/Choc etc.) not necessary?

• Too expensive

• E. coli was already isolated

• We were using only haemolytic bacteria

• We were using viruses