Restriction Enzymes and Vectors

1. If the recognition sequence of the restriction enzyme HindIII is AAGCTT, then how many covalent bonds will be broken by the enzyme in the following DNA molecule?

• 1

• 2

• 3

• 4

Who discovered Restriction Enzymes?

• a).Watson and Crick

• b).Arber ,Natha, Linn

• c).Jacob and Monod

• d).Beadle and Tatum

1. The DNA base sequences recognized by Endonuclease are called

• a).Restriction Site

• b). Recognition Site

• c).Fragmentation Site

• d).cutting Site

The sequence that that restriction Enzymes recognizes contains how many bases?

• a).1

• b). 3

• c).6

• d).12

Which was the first Restriction Enzyme discovered ?

• a).HindII

• b). EcoRI

• c).BamHI

• d).HindIII

How do we name restriction Enzymes ?

• a).First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

• b). First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

• c). First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

• d). First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

The recognition sites for restriction enzymes are :

• a).Tautomer

• b). Pallindrome

• c).Metamere

• d).Acronym

Restriction Enzymes are isolated from :

• a).Protozoa

• b). Bacteria

• c).Virus

• d).Fungi

Restriction Enzymes were primarily used as :

• a).Defence by Protozoa against antibiotics

• b).Tool by Virus to cleave Bacterial genome

• c)Defence by Bacteria against bacteriophages viruses

• d).All of the above

Restriction enzymes belong to class of enzymes:

• a).Peptidase

• b).Amylases

• c).Lipases

• d).Nucleases