Recombinant DNA Technology

What is the enzymatic function of restriction enzymes?

• To add new nucleotides to the growing strand of DNA.
• To join nucleotides during replication.
• To join nucleotides during transcription.
• To cleave nucleic acids at specific sites.

The unpaired nucleotides produced by the action of restriction enzymes are referred to as

• sticky end.
• base sequence.
• single strands.
• restriction fragments.

A ____________ is required to transfer genes from one organism to another.

• vector
• reverse transcriptase
• transport molecule
• genetic probe

What is a cloning vector?

• The DNA probe used to locate a particular gene in the genome.
• An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.
• The laboratory apparatus used to clone genes.
• An enzyme that cuts DNA into restriction fragments.

What are the typical characteristics of a cloning vector?

• Bacterial cells cannot survive without it when grown under certain conditions.
• It contains restriction sites that allow the insertion of foreign DNA segments.
• It can replicate in bacterial cells.
• All of the above.

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

What enzyme forms covalent bonds between restriction fragments?

• DNA primase
• DNA helicase
• DNA ligase
• DNA polymerase

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA

• I, II, IV, III, V
• III, II, IV, V, I
• II, III, V, IV, I
• III, IV, V, I, II

 Which of the following statement about plasmid is correct?
I ) It can replicate by itself
II ) Hybrid plasmid is introduced into bacteria by transformation
III ) Other than bacteria, plasmid can also be found in bacteriophage

• I only
• I and II only
• II and III only
• I,II and III

An organism in which foreign genes have been incorporated is called a:

• recombinant organism
• transgene recombinant
• polymorphism
• transgenic organism