Gene Expression and Cloning

What is the process of DNA replication?

• DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules.

• DNA replication is the process by which a double-stranded RNA molecule is copied to produce two identical RNA molecules.

• DNA replication is the process by which RNA is copied to produce two identical RNA molecules.

• DNA replication is the process by which a single-stranded DNA molecule is copied to produce two identical DNA molecules.

What does PCR stand for?

• Protein Chain Reaction

• Polymerase Chain Reaction

• Polymerase Chain Replication

• Polymerase Chain Reagent

What is the purpose of PCR?

• PCR is used to analyze protein structures.

• PCR is used to measure gene expression levels.

• PCR is used to create recombinant DNA.

• PCR is used to amplify a specific segment of DNA.

What is recombinant DNA?

• Recombinant DNA is a type of DNA created by duplicating the same DNA sequence.

• Recombinant DNA is a type of DNA created by combining DNA from different sources.

• Recombinant DNA is a type of DNA created by mutating the DNA sequence.

• Recombinant DNA is a type of DNA created by combining RNA from different sources.

Tags

NGSS.HS-LS3-2

What are cloning vectors?

• DNA molecules used to carry foreign RNA fragments into host cells for replication and amplification.

• Proteins used to carry foreign DNA fragments into host cells for replication and amplification.

• RNA molecules used to carry foreign DNA fragments into host cells for replication and amplification.

• DNA molecules used to carry foreign DNA fragments into host cells for replication and amplification

What are restriction enzymes?

• Enzymes that repair DNA damage.

• Enzymes that cut DNA at specific recognition sites

• Enzymes that synthesize DNA.

• Enzymes that bind to DNA and prevent transcription.

Which enzyme is responsible for joining DNA fragments together during cloning?

• DNA polymerase

• RNA ligase

• Restriction enzyme

• DNA ligase

What is the purpose of gel electrophoresis in molecular biology?

• To determine the sequence of DNA

• To separate and analyze DNA, RNA, or proteins based on their size and charge.

• To amplify DNA sequences

• To visualize protein-protein interactions

What is the role of a plasmid in cloning?

• The role of a plasmid in cloning is to prevent the replication of foreign DNA fragments.

• The role of a plasmid in cloning is to provide energy for the replication process.

• The role of a plasmid in cloning is to serve as a vector for carrying and replicating foreign DNA fragments.

• The role of a plasmid in cloning is to serve as a template for DNA synthesis.

What is the purpose of a primer in PCR?

• To provide a starting point for DNA synthesis

• To amplify the DNA sample.

• To denature the DNA strands.

• To provide a template for RNA synthesis.