SECTION B QUIZ ON PROCESSES OF RECOMBINANT DNA TECHNOLOGY

In case of bacteria which ezyme is used for Cell lysis

• chitinase

• lysozyme

• EDTA+SDS

• cellulase

Answer explanation

Cell lysis is the process in which outer membrane of cell or cell membrane is broken /disrupted by using enzyme for the extraction of DNA/RNA

a)   In case of Bacteria: -

·       Lysozyme enzyme is used for degradation of cell wall of bacteria.

b) EDTA (Ethylenediaminetetraacetic acid) which is a chelating agent which is used to destabilize the plasma membrane.

c) SDS  (Sodium Dodecyl Sulphate) is used as an detergent for solubilizing the plasma membrane.

d) In case of fungus chitin is used and plant cell wall is degraded with help of cellulase enzyme.

What is the enzymatic function of restriction enzymes?

• To join nucleotides during replication.

• To add new nucleotides to the growing strand of DNA.

• To join nucleotides during transcription.

• To cleave nucleic acids at specific sites.

What is the most logical sequence of steps for processes involved in recombinant DNA technology

1 . Isolation of the Genetic Material (DNA).

2.  Cutting of DNA at Specific Locations.

3.   Amplification of Gene of Interest using PCR.

4. Insertion of Recombinant DNA into the Host Cell/Organism.

5. Obtaining the Foreign Gene Product.

6. Downstream Processing.

• 1,2,3,4,5,6

• 6,3,2,1,5,4

• 4,5,1,3,2,6

• 6,5,4,3,2,1

Protease is used to remove which contaminant in Enzymatic treatment

• RNA

• DNA

• Ligase

• Protein

What is used in purification of DNA/RNA

• SDS

• RNAse

• Chilled Ethanol

• EDTA

What are the requirements for PCR

• Template DNA

• Forward and reverse Primer

• DNA Polymerase I

• Substrate

• all of the above

What is the sequence of PCR?

1. ANNELING

2. DENATURATION

3. EXTENTION

• 1,2,3

• 3,1,2

• 2,3,1

• 2,1,3

The recombinant DNA bearing gene for resistance to an antibiotic is transferred into ___________

• DNA

• E.coli

• Thermus Aquaticus

• none of the above

This process includes separation and purification called as_____

• DNA Isolation

• upsteam processing

• downstream processing

• purification of DNA

Electrophoresis is a separation technique used to separate DNA, RNA or Protein molecules based on their ____

• size

• electrical charge

• BOTH size and electrical charge

• NONE