Recombinant DNA Technology

The following are the uses of

recombinant DNA EXCEPT

• Produce sheep clone

• Produce plants that have bacterial genes to destroy pathogenic insects

• Produce agriculturally important plants and animals

• Produce microbes that secrete enzymes to overcome the problems of oil spoilage in the sea

A technique for making millions of copies of a specific region of DNA.

• DNA ligase

• gel electrophoresis

• restriction enzymes

• polymerase chain reaction

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.

• To create DNA nucleotides

• To copy DNA.

• To maintain the temperature of the PCR reaction.

Recombinant DNA molecule can be introduced into the host cell using

• Immunochemical methods

• PCR

• Transduction

• Protein interaction

The first recombinant DNA molecule was produced in

• 1972

• 1975

• 1977

• 1980

Vector carrying gene of interest is called as

• Vehicle

• Chimera

• Ligase

• restriction enzyme

Homopolymer tailing technique can be used to

• isolate DNA fragments

• Joining vector and insert

• introduction into the host cell

• screening of recombinants

Scientists can use a restriction enzyme to cut open a bacterial plasmid and insert a human gene into it. This forms ______. (page 181)

• DNA fingerprints

• binary fission

• superplasmids

• recombinant DNA

Write a paragraph describing the benefit that would come from producing the genetically modified organism illustrated here.

Tags

NGSS.HS-LS2-7

Gene therapy often uses weakened ______ to transport genes. (Page 180)

• viruses

• bacteria

• stem cells

• vaccines