Column Chromatography

Determine which statement is true:

• This is a chromatogram of a pure substance

• In this chromatogram all of the substances have relatively equal attractions to the stationary phase

• This is a chromatogram of a mixture with three components having equal amounts in the mixture

• This is a mixture with three components that each have different amounts present in the sample

Which ink sample looks like a pure substance. (Use the letters below the baseline to identify the inks)

• Bc

• Br

• G

• O

Tags

NGSS.HS-PS1-3

What is the retention time of the peak present in the greatest quantity?

• 3.5 minutes

• 4.1 minutes

• 4.5 minutes

• 7.7 minutes

Which is the correct equation for calculating an Rf value?

• Distance of Mobile phase/distance of substance

• Time in mobile phase/time is stationary phase

• distance of substance/distance of mobile phase

• Baseline/solvent front

In column chromatography, the sample is added to the top of the column and gravity pulls it through the resin bead matrix. The molecules are separated based on their size, shape, or charge. What is this process called?

• Filtration

• Dialysis

• Fractionation

• Diffusion

Column chromatography involves adding the sample to the top of the column. The sample is then pulled through the resin bead matrix by gravity. This process is known as ________.

• Filtration

• Fractionation

• Dialysis

• Diffusion

What is the purpose of the 'frit' in a chromatography column?

• To separate molecules by size

• To hold the resin in place

• To visualize molecules

• To add color to the sample.

In ion-exchange chromatography, if a protein's charge is unknown, its behavior in the column may change depending on the pH of the solution. This is because the protein's charge can be _______.

• Positive

• Negative

• Neutral

• Variable

In a chemistry lab, Henry, Benjamin, and Priya are working on an experiment. They need to identify the __________ that moves analyte through the column into the detector.

• Mobile Phase

• Stationary Phase

• Liquid Phase

• Liquified Phase

Answer explanation

In the given question, Henry, Benjamin, and Priya are trying to identify the component that moves analyte through the column into the detector in a chemistry lab experiment. The correct answer is 'Mobile Phase'. This is because in chromatography, the mobile phase is the medium that carries the mixture of substances to be separated through the column to the detector.

Every component have differential affinity, chemical structure & _________temperature when interact with column, thus separation occur at specific retention time

• Boiling Point

• Freezing Point

• Cloud Point

• Cut Point

Answer explanation

In the given question, the missing term is related to temperature when components interact with a column. Boiling Point is the correct choice because it refers to the temperature at which a substance changes from liquid to gas, affecting its interaction with the column and resulting in separation at specific retention times.