Genomics

The principle of Sanger's method relies on

• use of chemicals for base specific cleavage

• use of dNTPs for chain termination

• use of ddNTPs for chain termination

• Use of p32 for chain termination

NGS consists of the following steps in sequence

• library construction, amplification, sequencing

• sequencing, library construction, amplification

• Amplification, Sequencing and library construction

• none of the above

Emulsion PCR refers to

• amplification of DNA in water

• amplification of DNA in organic solvent

• amplification of DNA in water in oil droplets

• synthesis of DNA in water

emulsion PCR can be linked to

• Solid sequencing

• illumina sequencing

• 454 sequencing

• automated sanger sequencing

Which of these characters kickstarts a genomics project without a biological question or sound hypothesis?

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Enzymes that cut DNA molecules when they identify specific nucleotide sequences.

• gel electophoresis

• plasmid

• restriction enzymes

• cloning

An electrical current is used to separate a mixture of DNA fragments from one another.

• Cloning

• gene sequencing

• restriction map

• gel electrophoresis

These show the length of DNA fragments between restriction sites in a strand of DNA.

• restriction maps

• cloning

• gene sequencing

• bioinformatics

A technique that produces millions or even billions of copies of a specific DNA sequence in just a few hours.

• gene sequencing

• PCR

• DNA microarray

• cloning

DNA that contains genes from more than one organism.

• recombinant DNA

• restriction enzyme

• plasmid

• DNA microarray