Enzymes and pH

Choose two enzymes. First, describe one way the two enzymes are different. Then, describe one way they are the same.

Stem: I choose ___ and ____. ___ while ____ . Both enzymes are ____.

WORD BANK: best pH, function, pepsin, salivary amylase, phosphatase, optimal pH, acidic environment, basic environment

What are some factors that can affect the thermal stability of enzymes?

• Light intensity, pressure, humidity

• Salt concentration, enzyme concentration, reaction time

• Enzyme size, reaction volume, stirring speed

• pH, temperature, substrate concentration, inhibitors or activators

A type of protein that speeds up a chemical reaction in a living thing

• enzyme

• anabolic reactions

• catabolic reactions

• biochemistry

How does pH affect enzyme activity?

• Enzymes are not affected by pH

• Enzymes work best at a specific pH range

• Enzymes are only active at neutral pH

• Enzymes are more active at high pH

What is the optimal pH for this enzyme?

• 2

• 4

• 8

• 12

Which of the following conclusions can be drawn from this graph?

• The enzyme’s activity is greater around pH of 8.0 than around pH of 5.0.

• The enzyme’s activity continually increases as pH increases from 5.0 to 9.0

• The optimum pH of the enzyme is 5.8

• A. The optimum pH of the enzyme is 6.6.

What is the effect of extreme pH levels on enzyme structure?

• Enzymes become more efficient

• Enzymes become denatured

• Enzymes are unaffected

• Enzymes change their substrate

Which of the following statements is true about enzyme activity?

• Enzyme activity decreases with increasing substrate concentration indefinitely

• Enzyme activity is independent of temperature

• Enzyme activity can be affected by both temperature and pH

• Enzyme activity is not affected by environmental conditions

What is the optimal pH range for most human enzymes?

• A) 1-3

• B) 4-6

• C) 6-8

• D) 9-11

Which enzyme is most active in acidic conditions?

• A) Pepsin

• B) Amylase

• C) Trypsin

• D) Lipase