How does the guide RNA help the cell know where to cut the DNA?

• Guide RNA helps to find the PAM

• Guide RNA is complementary to the DNA sequence

• Guide RNA always binds to the same sequence of DNA

• Like restriction sites, they are always palindromes

The enzyme portion of CRISPR??- this process is called ________.

or What enzyme is used in CRISPR gene editing?

• CRISPR

• Replicase

• Lysozyme

• Cas9

What does the cas9 protein do?

• cut viruses

• Cut RNA

• cut DNA

• ingest bacteria

What does the guide RNA do?

• destroy invaders like bacteriophages immediately

• cut invading pieces of RNA

• recognize invaders

• recognize sequences of DNA to be edited.

What is the role of gRNA? (gRNA -> guide RNA)

• the cell recognizes that DNA is damaged and tries to repair it

• ensures that the Cas9 enzyme cuts at the right point in the genome

• use the DNA repair machinery to introduce changes to one or more genes

• makes a cut across both strands of the DNA

What does CRISPR stand for?

• Clustered regularly interactive short palindromic repeats

• Clustered regularly interspaced short palindromic repeats

• Deoxyribonucleic acid

• Regularly interspaced short palindromic repeats

CRISPR-Cas9 was discovered in which of the following organisms?

• protists

• fungi

• bacteria

• viruses

What did scientists look at to help them create CRISPR?

• Sheep cloning

• Palindromic repeats

• Common Cold

• Bacteria fighting off viruses

What is one way CRISPR has already been used to change genes?

• Clone sheep

• Give people superpowers

• Cure animal diseases

• Military purposes

How does CRISPR differ from previous techniques of gene editing?

• slower, more expensive, but more accurate

• faster, cheaper, and more accurate

• faster, more expensive, more accurate

• slower, cheaper, less accurate