MI

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

• positive, negative

• positive, positive

• negative, positive

• negative, negative

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

• faster than

• at the same rate as

• slower than

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

Place the steps of DNA Sequencing below into their correct order.

• A, B, C, D

• B, C, A, D

• B, A, C, D

• D, B, C, A

Which of the following base pairs is matched correctly?

• Guanine and Thymine

• Cytosine and Adenine

• Adenine and Thymine

• Thymine and Cytosine

What is the purpose of Polymerase Chain Reaction (PCR) in the process of sequencing DNA?

• To separate DNA fragments by size

• To synthesize copies of template DNA

• To change the structure of template DNA

• To terminate the elongation of a DNA strand

How is DNA synthesis in PCR and Cycle Sequencing DIFFERENT?

• Only PCR uses deoxynucleotides.

• Cycle Sequencing generates DNA products of different lengths.

• Only PCR analyzes DNA products using gel electrophoresis.

• Only Cycle Sequencing uses dideoxynucleotides.

Write the correct sequence that matches this chromatogram.

What is the primary function of mitochondria in eukaryotic cells?

• Energy production through ATP synthesis

• Protein synthesis

• Photosynthesis

• DNA replication

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