DNA Fingerprinting and Gel Electrophoresis

Gel electrophoresis separates fragments of DNA by:

• Size/weight

• Genes

• Base pairs

• Electromagnetic charge

In a DNA fingerprint, lighter-weight fragments can be found at the:

• Top

• Bottom

• Middle

• Front

Which suspect left the blood stain?

• Bob

• Sue

• John

• Lisa

Which suspect matches the crime scene?

• Suspect 1

• Suspect 2

• Neither

Tags

NGSS.HS-LS3-2

Imagine that you have a sample of DNA that contains a single, specific DNA sequence. Before you run your gel, you split your sample into two tubes. You run the DNA from the first tube and use an enzyme called a restriction enzyme that cuts the DNA into two pieces at a specific point in the sequence. You then run this sample that was cut on the gel. Which if the next three lanes could be the same sample from lane two after it was cut into two pieces?

• 3

• 4

• 5

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are

The speed at which DNA moves through a gel is diretly related to its size.

• True

• False

How many distinctive markers are used for DNA fingerprinting to distinguish between two people?

• 10

• 13

• 15

• 20

Tags

SFS3

The parents of a new baby believe they brought the wrong child home from the hospital. Gel electrophoresis was performed using DNA samples from the parents and the child. A section of the gel electrophoresis results is shown below. Which conclusion is valid based on the gel electrophoresis results?

• They have the correct child, because her genetic information is identical to that of the father.

  
  

• They have the wrong child, because her genetic information does not match that of either parent.

• They have the correct child, because her genetic information came from both parents.

  
  

• They have the wrong child, because her genetic information matches only that of the mother.

Tags

NGSS.HS-LS3-1

DNA fingerprinting is a technique used to:

• Identify an individual based on their unique genetic profile

• Determine the hair color of a person

• Analyze fingerprints left at a crime scene

• Decode an individual's entire DNA sequence

Tags

NGSS.HS-LS3-2