Mi 1.1 Elisa

In an ELISA assay, ________ and ________ are added to facilitate a color change.

• antibodies and antigens

• primary antibodies and secondary antibodies

• enzymes and substrates

• antigen and enzymes

A sample of spinal fluid is taken from a college student suspected to have contracted spinal meningitis.Which diagnostic test will confirm the diagnosis fastest?

• Culturing the spinal fluid and testing for bacterial colony growth in a Petri dish.

• An ELISA Assay to determine the presence of menigitis antigens.

• DNA sequencing

• Protein Chromatography

What does ELISA stand for?

• E coli Immune System Antigen

• Enzyme-linked Immunosorbent Assay

• Enzyme Leuko-immune Standard Assessment

• Estimated Lymph Integrated System Assessment

How the ELISA used in class both a quantitative and a qualitative test?

• ELISA yields a plus or minus sign for presence of antigen and a number for amount present.

• ELISA yields a color change to indicate the presence of the antigen and a generates a number for the amount of antigen present

• ELISA yields a color change for presence of antigen and the intensity of the color indicates the amount present

• There is a computer printout with the results of the test and the amount present

A doctor orders an ELISA test for two patients. The results show a color change from clear to blue. Patient A results are a dark blue while the results for patient B are a light blue. What is the most likely diagnosis?

• Patient A is more infected

• Patient B is more infected

• The patients are infected equally

• Neither patient is infected

________ have surface _________ where _______ attach

• pathogens, antigens, antibodies

• antigens, antibodies, pathogens

• pathogens, antibodies, antigens

• antibodies, antigens, pathogens

When a positive + result occurs in an ELISA test, then one could conclude that...

• a + result always means illness

• the antibody is present

• it is a false + and no illness

• an enzyme is present

Why is it necessary to wash the samples repeatedly during an ELISA protocol?

• it is important to keep the lab wells clean

• it is important to wash away all bound and unbound antibodies from teh wells

• it is important to wash away the unbound antibodies

• washing the wells is just solid lab practice

What is the primary purpose of an ELISA test in a laboratory setting?

• To detect the presence of specific antigens or antibodies

• To measure the pH level of a solution

• To determine the boiling point of a liquid

• To identify the genetic sequence of a virus

Which component in an ELISA test is responsible for producing a color change?

• The substrate

• The antigen

• The antibody

• The buffer solution