Quiz on Serial Dilution and Titer Calculation

What is the first step in preparing the test tubes for the serial dilution?

• Add 0.25 ml of antibody A to tube #1

• Centrifuge the tubes for 20 seconds

• Add 0.5 ml of saline to each tube

• Label the test tubes 1-10

How much saline is placed in each of the ten test tubes?

• 2 ml

• 0.25 ml

• 1 ml

• 0.5 ml

What is the purpose of mixing the solution in the micropipette three times?

• To create bubbles

• To ensure proper mixing

• To increase the volume

• To cool the solution

What should be done with the solution in tube #10 after the dilution process?

• Add more saline

• Discard 0.25 ml

• Centrifuge for 20 seconds

• Label it as the endpoint

What is added to each tube after the saline and antibody A?

• 0.1 ml of serum

• 0.5 ml of saline

• 0.25 ml of distilled water

• 0.25 ml of 3% red blood cell suspension

How long should the tubes be placed in the refrigerator?

• 10 minutes

• 30 minutes

• 1 hour

• 2 hours

What is the next step after removing the tubes from the refrigerator?

• Mix the tubes again

• Add more saline

• Centrifuge for 20 seconds

• Label the tubes

What does the endpoint of the test indicate?

• The minimum dilution that gives no reaction

• The maximum dilution that gives visible agglutination

• The total volume of the solution

• The amount of antibody used

What is the formula for calculating dilution?

• Dilution = Amount of Solute - Total Volume

• Dilution = Amount of Solute + Diluent

• Dilution = Total Volume / Amount of Solute

• Dilution = Amount of Solute / Total Volume

In a 1:20 dilution, how many parts of diluent are used?

• 1 part

• 19 parts

• 5 parts

• 20 parts