11BIO Inquiry Task 1: Wet Mount Preparation

Which formula is used to calculate total magnification of a microscope?

• Eyepiece magnification ÷ objective magnification

• Eyepiece magnification × objective magnification

• Objective magnification × 10

• Eyepiece magnification + objective magnification

If a microscope has an eyepiece lens of 10× and an objective lens of 40×, what is the total magnification?

• 400×

• 40×

• 50×

• 100×

What happens to the image when total magnification increases?

• The image becomes smaller and clearer

• The image appears larger but may lose some clarity

• The field of view increases

• The resolution always improves

Which part of the microscope is adjusted to change the magnification?

• The coarse focus knob

• The eyepiece lens

• The objective lens

• The diaphragm

What does ‘Field of View (FOV) diameter’ refer to in microscopy?

• The total magnification of the image

• The area visible through the microscope, measured across its widest point

• The thickness of the specimen

• The sharpness of the image

If the FOV diameter at low power (40× total magnification) is 2 mm, what is this value in µm?

• 20 µm

• 200 µm

• 2000 µm

• 20000 µm

Which formula is used to calculate the FOV diameter at higher power?

• FOV at higher power = FOV at lower power × (low power / high power)

• FOV at higher power = FOV at lower power × high power

• FOV at higher power = FOV at lower power ÷ low power

• FOV at higher power = FOV at lower power × (high power / low power)

If the FOV diameter at low power (40× total magnification) is 2000 µm, what is the FOV diameter at high power (400× total magnification)?

• 2000 µm

• 200 µm

• 20 µm

• 2 µm

A specimen takes up one-third of the FOV diameter at 200 µm. What is its estimated size?

• 600 µm

• 66.7 µm

• 133.3 µm

• 100 µm

Why is FOV diameter important when estimating cell size?

• It allows you to measure cell size by counting how many fit across the FOV

• It tells you how bright the specimen will appear

• It determines the total magnification

• It helps you focus on the specimen