Molecular Biology Techniques Quiz

What is the primary function of DNA polymerase in PCR?

• To separate DNA strands

• To synthesise new DNA strands

• To cut DNA at specific sites

• To ligate DNA fragments

Which of the following is a key principle of PCR?

• DNA denaturation, annealing, and extension

• DNA methylation, transcription, and translation

• DNA replication, transcription, and translation

• DNA ligation, transformation, and cloning

What is the main application of gel electrophoresis?

• To amplify DNA sequences

• To separate DNA fragments by size

• To synthesise RNA from DNA

• To cut DNA at specific sequences

DNA probes are primarily used for:

• Amplifying DNA sequences

• Detecting specific DNA sequences

• Cutting DNA at specific sites

• Ligating DNA fragments

What is the role of DNA primers in PCR?

• To initiate DNA synthesis

• To cut DNA at specific sites

• To ligate DNA fragments

• To separate DNA strands

Which enzyme is used to cut DNA at specific sequences?

• DNA polymerase

• DNA ligase

• Restriction enzymes

• Reverse transcriptase

What is the function of DNA ligase?

• To synthesise new DNA strands

• To cut DNA at specific sites

• To join DNA fragments together

• To separate DNA strands

Reverse transcriptase is used to:

• Convert RNA into DNA

• Convert DNA into RNA

• Cut DNA at specific sites

• Ligate DNA fragments

In PCR, what is the purpose of the annealing step?

• To separate DNA strands

• To allow primers to bind to the DNA template

• To synthesise new DNA strands

• To cut DNA at specific sites

Which of the following is NOT an application of gel electrophoresis?

• DNA fingerprinting

• Protein separation

• DNA sequencing

• DNA synthesis