Topic 9: Enzymes as Catalysts

Which of the following is a type of catalysis involving the formation of a covalent bond between the enzyme and the substrate?

• Specific acid/base catalysis

• Covalent/nucleophilic catalysis

• Electrophilic catalysis

• General acid/base catalysis

What type of catalysis involves the donation or acceptance of a proton by the enzyme?

• Covalent/nucleophilic catalysis

• Electrophilic catalysis

• Specific acid/base catalysis

• Mandelate racemase

Which enzyme is known for its role in the conversion of carbon dioxide and water to bicarbonate and protons?

• Mandelate racemase

• Carbonic anhydrase

• Lysozyme

• Chymotrypsin

Which enzyme is involved in the breakdown of bacterial cell walls?

• Carbonic anhydrase

• Lysozyme

• Mandelate racemase

• Proteases

Which of the following is an example of a protease enzyme?

• Mandelate racemase

• Lysozyme

• Chymotrypsin

• Carbonic anhydrase

What determines the substrate specificity of serine proteases?

• Catalytic triad

• Binding pocket

• Intermediate formation

• Transition state stabilization

What is the common feature of all serine proteases?

• Catalytic triad of Ser, His, Asp

• Acid base catalysis

• Redox reactions

• Formation of a covalent bond

What type of catalysis is described by Step 1

• General Acid Catalysis

• General Base Catalysis

• Specific Acid Catalysis

• Specific Base Catalysis

What type of catalysis is described by Step 2

• General Acid Catalysis

• General Base Catalysis

• Specific Acid Catalysis

• Specific Base Catalysis

Blank occurs when the enzyme active site stabilizes the transition state of the reaction by forming electrostatic interactions with the substrate. The electrostatic interactions can be ionic, ionic-dipole, dipole-dipole, or hydrophobic interactions. Hydrogen bonding is one of the most common electrostatic interactions formed in the active site.