Enzymes in DNA Manipulation Quiz

What is the primary function of DNA polymerase in DNA manipulation?

• To cut DNA strands

• To synthesise new DNA strands

• To join DNA fragments

• To degrade DNA

Which enzyme is used to join two DNA fragments together?

• Endonuclease

• Ligase

• Polymerase

• Reverse transcriptase

What is the role of endonucleases in DNA manipulation?

• To synthesise DNA

• To join DNA fragments

• To cut DNA at specific sites

• To convert RNA into DNA

What is the difference between blunt ends and sticky ends in DNA fragments?

• Blunt ends have overhanging bases, sticky ends do not

• Sticky ends have overhanging bases, blunt ends do not

• Both have overhanging bases

• Neither have overhanging bases

Which enzyme is used to synthesise DNA from an RNA template?

• DNA polymerase

• Ligase

• Reverse transcriptase

• Endonuclease

What is the main advantage of using Taq polymerase in PCR?

• It can cut DNA at specific sites

• It is heat-stable and can withstand high temperatures

• It joins DNA fragments

• It degrades RNA

Which of the following is a characteristic of sticky ends?

• They are single-stranded overhangs

• They are double-stranded

• They do not have overhangs

• They are circular

What is the purpose of using probes in DNA manipulation?

• To synthesise DNA

• To join DNA fragments

• To identify specific DNA sequences

• To degrade DNA

How does Taq polymerase differ from regular DNA polymerase?

• Taq polymerase is not heat-stable

• Taq polymerase is heat-stable

• Taq polymerase can cut DNA

• Taq polymerase can join DNA fragments

Which enzyme would you use to cut DNA at a specific sequence?

• Ligase

• Endonuclease

• Reverse transcriptase

• DNA polymerase