Sterilization and Disinfection

________ is the elimination of all life forms on an object.

• antisepsis

• disinfection

• pasteurization

• sterilization

Which of the following is a brief period of heat to reduce the number of microorganisms?

• antisepsis

• disinfection

• pasteurization

• sterilization

Living tissue can be sterilized.

• True

• False

Who first used carbolic acid and other aseptic techniques during surgery?

• Ignaz Semmelweis

• Joseph Lister

• Louis Pasteur

• Robert Hooke

Which of the following men is a handwashing pioneer?

• Ignaz Semmelweis

• Joseph Lister

• Louis Pasteur

• Robert Hooke

When selecting an antimicrobial procedure, you should keep in mind:

• The type and number of microorganisms present.

• Environmental conditions.

• Object being treated.

• All of the above should be considered.

Tags

NGSS.HS-ETS1-3

Which of the following is LEAST resistant to antimicrobial procedures?

• capsule formers

• endospores

• enveloped viruses

• mycolic acid layer

Moist heat:

• includes boiling, pasteurization, and autoclaving.

• does not destroy endospores and heat stable toxins.

• denatures proteins, including critical enzymes, within the microorganism.

• All of the above are true of moist heat.

What are the parameters at which we run an autoclave for a 500 ml flask of media?

• 20 minutes, 15 psi > atmospheric pressure, 150 degrees Celcius

• 15 minutes, 20 psi > atmospheric pressure, 121 degrees Celcius

• 15-20 minutes, 15 psi > atmospheric pressure, 121 degrees Celcius

• 1 hour, 15 psi > atmospheric pressure, 121 degrees Celcius

Dry heat is a method of sterilization often used in the microbiology laboratory.

• True

• False