Sequence Alignment Quiz

What is pairwise sequence alignment?

• Comparing two sequences to identify similarity

• Aligning three or more sequences

• Visualizing protein structure

• Calculating gene expression levels

What is the difference between global and local alignment?

• Global aligns full sequences; local aligns regions of highest similarity

• Local aligns entire genome; global doesn't

• Local uses 3D structure; global uses sequence

• Global is only for RNA

In which scenario is global alignment most appropriate?

• When comparing two sequences of similar length with overall high similarity

• When comparing a short motif within a long genomic sequence

• When searching for conserved protein domains

• When aligning only the regions with highest identity

When would local alignment be preferred over global alignment?

• When identifying conserved regions within sequences of different lengths

• When it is of importance to identify every single difference between two full-length sequences

• When comparing two protein sequences of the same size

• When the sequences are nearly identical throughout

What is BLAST used for?

• Finding sequences that are similar to a query sequence from the Genbank

• Predicting protein folding

• Calculating GC content

• Aligning entire genomes

Which type of alignment does BLAST use?

• Local alignment

• Global alignment