Enzymes and Cell Disruption Quiz

Where are enzymes commonly found in prokaryotic cells?

• Nucleus

• Cytosol

• Cell wall

• Periplasmic space

The simplest method of cell disruption is:

• Homogenization

• Sonication

• Osmotic shock

• Detergent treatment

Which type of detergent is most commonly used in bioseparation?

• Ionic

• Anionic

• Non-ionic

• Cationic

What is the structure that solubilized lipids form in aqueous solution?

• Bilayer

• Liposome

• Micelle

• Vesicle

A major drawback of enzymatic digestion for cell lysis is:

• It is non-specific

• It denatures proteins

• It is expensive

• It cannot be scaled up

Homogenization disrupts cells using:

• Chemical lysis

• Osmotic pressure

• Shear stress and cavitation

• Electrical impulses

One of the main disadvantages of homogenization is:

• Unpredictable results

• Excessive foaming

• High heat generation

• Low throughput

Bead mill is particularly effective for disrupting:

• Mammalian cells

• E. coli

• Yeast and fungi

• Algae with thin walls

Organic solvents disrupt cells by:

• Dehydrating the membrane

• Dissolving membrane lipids

• Destroying proteins

• Denaturing DNA

A drawback of using organic solvents is:

• High pressure requirement

• Difficulty in heating

• Toxicity and product recovery challenges

• Limited to Gram-negative bacteria